Journal: Cell

Article Title: Serum Amyloid A Proteins Induce Pathogenic T H 17 Cells and Promote Inflammatory Disease

doi: 10.1016/j.cell.2019.11.026

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Flow Cytometry: anti-mouse MHCII (M5/114.15.2) PerCP-cy5.5 , BD Bioscience , Cat. 562363.

Techniques: Flow Cytometry, Western Blot, In Vitro, Cell Differentiation, Activation Assay, In Vivo, Immunohistochemistry, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, SYBR Green Assay, Ex Vivo, Isolation, Knock-In, Knock-Out, Software

Journal: Nature immunology

Article Title: γδ T cells producing interleukin-17A regulate adipose regulatory T cell homeostasis and thermogenesis

doi: 10.1038/s41590-018-0094-2

Figure Lengend Snippet: a, Representative flow cytometry plots of γδ T cells from the epididymal WAT stromal vascular fraction (eWAT SVF). K denotes thousand. b, Frequency of γδ T cells across various adipose tissue depots as a percentage of CD3ε+ T cells in male eWAT (n ≥ 4 mice per group). Each symbol represents an individual mouse; small horizontal lines indicate the mean. c, Flow cytometry of lymphocytes in the blood, liver, spleen, and adipose tissue of CD45.1+ and CD45.2+ congenic C57BL/6 parabiotic pairs joined at 6 weeks of age and analyzed 2 weeks later (left); and frequency pie charts of CD45.1+ and CD45.2+ CD8+ and γδ T cells (right). d, Frequency of Vδ1+, Vδ2+, and Vδ3+ γδ T cells (% of CD45+PI− cells) in peripheral blood (PBMCs) or from matched omental fat from patients before bariatric surgery. Data are representative of three experiments (a,b,d; mean ± s.e.m. in b) or one experiment (c).

Article Snippet: Anti-TCR Vδ1 (REA173) and anti-TCR Vδ2 (123R3) were purchased from Miltenyi Biotec.

Techniques: Flow Cytometry